Advanced rDNA
Yeast Two-Hybdrid (Y2H) system explained
The Yeast Two-Hybrid (Y2H) system or Yeast Two-Hybrid Assay represents a powerful in vivo technique for detecting and analyzing protein-protein interactions, where the physical association…
Ligase Chain Reaction (LCR) Explained
Ligase chain reaction (LCR) is a thermostable DNA ligase-dependent DNA amplification which can be initiated by repetitive cycles of the ligation of adjacent hybridized DNA…
Bacterial artificial chromosomes (BACs)
Bacterial artificial chromosomes (BACs) are single-copy-number, high-capacity circular DNA vectors derived from Escherichia coli fertility factor (F-plasmids); they can accommodate approximately 350 kb of inserted…
High-Capacity Yeast Artificial Chromosomes (YACs) Vectors
A Yeast Artificial Chromosome (YAC) is a shuttle high-capacity useful for the physical mapping of complex genomes and for the cloning of large genes. It…
Adenovirus viral vector (AVV) Guide
Adenovirus contains a 36-Kb linear dsDNA genome with ITRs at both ends and consists of early (E1-4) and late (L1-5) heavily spliced transcripts. Of the…
Protein expression in Pichia pastoris
Pichia pastoris has many of the advantages of higher eukaryotic expression systems such as protein processing, protein folding, and the availability of posttranslational modifications, while…
pBR322 Plasmid Vector: Introduction, Mapping, Applications, and Modifications
pBR322 is a circular, double-stranded DNA plasmid, 4 361 base pairs that contains important features such as antibiotic resistance genes for ampicillin (AmpR) and tetracycline…
Lambda EMBL3 and EMBL4 Vector Selection Using Spi- Phenotype
Lambda EMBL3 and EMBL4 are high-capacity vectors derived from bacteriophage lambda, used for genomic library construction. They utilize the Spi- phenotype selection method, which allows…
